Hughes had been working around the clock with Sixers' physio Danny Redrup since injuring the left ankle on Saturday night but fell short of playing today after a last hour fitness check.
The South Australian based, Western Australian-born 26-year-old left handed top order batter Jake Carder comes from the Player Replacement Pool into the the starting lineup to make his debut for the club.
Carder had not met many of his new teammates prior to the match and has never trained with the Sixers.
He made his Sheffield Shield debut for South Australia in round one of the tournament this season, scoring 118 in the first innings alongside Travis Head, Adam Carey and several of the Adelaide Strikers players he will oppose tonight.
In his second Shield match he scored 79 in the second innings and in his third appearance 68 in the second dig.